Figure 1. CME is not involved in baculovirus entry.

293 cells that were allowed to internalize baculovirus (wt) for 5 (A) and 15 min (B) were immunolabeled for baculovirus (red) and clathrin heavy chain (green). Colocalized voxels are shown in the inset. Scale bar, 10 µm. (C) Colocalization between baculovirus and clathrin heavy chain in 293 cells were calculated from confocal sections using the colocalization algorithm in the BioimageXD software (See Materials and Methods) from 3 separate samples, from 30 cells. Mean values and standard deviations are shown. (D) Differential labeling of intracellular (baculovirus Ab, Alexa488) and surface-bound baculovirus (baculovirus Ab, Alexa555) at 30 min was measured in dynasore-treated (Dyn, 80 µM) HepG2 cells from three separate samples (30 cells). Confocal sections were further analyzed by BioimageXD using the internalization algorithm. Mean values and standard deviations are shown.