Figure 5. TSA and AZA treatments do not affect cohesin recruitment.

(A) Hela cells grown for five days in the presence of 30 ng/mL of TSA, 1 micromolar AZA or no drug, were pre-extracted before fixation, fixed and stained with DAPI (blue) and antibodies against the three HP1 isoforms (green). Scale bar, 10 micrometers. (B) Immunoblot analysis of the chromatin fractions obtained from untreated and TSA- and AZA-treated cells, as described in Figure 4A. (C) Quantitation of Scc1-myc positive mitotic cells, as described in Figure 4D.