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Appearance of lipidated Tlc (NBD-cholesterol loaded) applied directly to the AT buffer surface (no lipids present). At 20°C, the film showed a banded appearance that was more intense at higher pressure. At 37°C, the film was very mobile and diffuse at low pressure, and at high pressure weakly fluorescent patches could be seen that were far less mobile. The brighter fluorescent dots might be crystals of free cholesterol.
