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∏-T curves for the adsorption of various forms of Tlc (purified, lipidated, delipidated, and relipidated) to a human Meibomian lipid film or a bovine Meibomian lipid film. The Meibomian lipid films were compressed to have ∏init = 10 mN/m before injecting proteins into AT subphase. These curves are compared with adsorption of purified Tlc to an air-AT interface (bottom curve, no lipid layer).
