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Penetration at 37°C of Tlc into a human Meibomian lipid film initially held at 10 mN/m. The micrographs show the appearance of the film before penetration (Pre) and at various times after penetration (4500 s, 13,000 s). After equilibrium was reached (32,500 s), the film was compressed and its appearance during (∏ = 25 mN/m) and at maximum compression (∏ = 41 mN/m) are shown.
