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. Author manuscript; available in PMC: 2009 Sep 19.
Published in final edited form as: Cell. 2008 Sep 19;134(6):981–994. doi: 10.1016/j.cell.2008.08.037

Figure 5. Analysis of resection and G2/M DNA damage checkpoint arrest in sgs1Δ exo1Δ cells.

Figure 5

(A) Position of two probes with respect to the DSB and EcoRI sites used to analyze 5’ strand processing in sgs1Δ exo1Δ cells. Southern blot analysis of 5’ strand resection in indicated mutants. Position of HO cut band (DNA fragment 1) and additional bands (DNA fragments 2 to 4) observed in sgs1Δ exo1Δ cells is indicated. (B) Plot demonstrating kinetics of resection in wild-type and sgs1Δ exo1Δ cells. Pixel intensities of the signal corresponding to the unprocessed HO cut band separately or added to the signal of the band(s) corresponding to paused degradation are presented. (C) Analysis of G2/M arrest after induction of the HO break in indicated mutants. (D) Number of cells forming Ddc2 foci after DSB induction was analyzed in indicated mutant and wild-type cells.