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. 2009 Mar 9;119(4):986–996. doi: 10.1172/JCI35814

Figure 3. Cfb is induced by LPS in cardiomyocytes.

Figure 3

(A) LPS induces Cfb transcripts in neonatal cardiomyocytes. RNA from cultured mouse neonatal cardiomyocytes was isolated after 12 hours of treatment with 10 μg/ml LPS, and qRT-PCR was performed to detect Cfb RNA expression. Results were normalized to Hprt, and the relative abundance of Cfb RNA is displayed. (B) LPS-induced increase in CFB protein in cultured neonatal cardiomyocytes. Cells were grown in serum-free medium and treated with LPS (10 μg/ml) for 24 hours. (C) Membrane damage caused by complement fixation in neonatal cardiomyocyte cultures from WT and Cfb–/– mice was determined by LDH leakage in the culture medium after LPS treatment. LDH activity after LPS treatment (control) or LPS treatment in the presence of mouse serum (serum) was compared. Ratios of background subtracted LDH activity in the culture medium and total cellular content (Triton X-100 lysates) were determined after 24 hours of LPS treatment. All experiments were done with at least 3 samples.