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Expression of test protein constructs in the wild-type, FMO1-deleted (Δfmo), FMO-rescued (pFMO), and HAC1-deleted (Δhac1) strains of yeast. The wild-type strain (DBY1827), transformed with the parent vector (pYES2), was used as the control. Expression of test proteins was induced for 6 hr at midlog phase (OD600 between 0.6 and 1.0) by adding 2% galactose, except bacterial alkaline phosphatase-expressing cells, which were automatically induced at late-log phase.
