Figure 5.

yFMO affects the duration of the Hac1p-mediated UPR. (a) The expression of chitinase as a function of time after induction in wild-type control cells with pYES2 (■) and those transformed with a chitinase expression plasmid (pGAL-chit) (●). (b) Western blot showing the time course of Hac1p expression in control cells with the parental vector, pYES2, and in wild-type, Δfmo, and Δhac1 strains bearing the expression plasmid (pGAL-chit). Chitinase expression was induced by 2% galactose; 20 μg of protein from cell extracts was loaded into the wells.