Table 1.
Experimental design for two internalized bacteria/control comparisons and four methods for calculating protein expression ratios. The variable ScRatio1 thus represents the ratio of internalized PG and PG controls grown under normal culture conditions (see 2.1) calculated using the protein level spectral count method. The other seven conditions are defined analogously according to their position in the table.
| PG_PP/PG_nm | PG_PP/PG_PPC | |||
|---|---|---|---|---|
| Protein level | Peptide level | Protein level | Peptide level | |
| Spectral count | ScRatio1 | ScRatio3 | ScRatio2 | ScRatio4 |
| Signal intensity | PiRatio1 | PiRatio3 | PiRatio2 | PiRatio4 |