Table 1.

Correlation between cytokine levels in bladder at time of animal sacrifice and in last voided urine specimen before sacrifice.

Cytokine	Last Voided Urine (pg/mg creatinine)	Tissue cytokine levels expressed per bladder (ng)	Spearman’s correlation coefficients	Fold change In CYP treated bladder over Vehicle treated bladder
GM-CSF	5.2±1.07	27.8±2.1	0.87	1
IL-1α	26.3± 11.5	47.1±13.1	0.5	2.5
MCP-1	121.6± 47.1	213.2±58.8	0.8	3.5
IL-4	64.9±48.2	35±3.28	0.97*	2
IL-1β	13.9±8.7	76.4±18.4	0.97*	4
IL-2	2.4±1.7	33.6±10.4	−0.05	0.5
IL-6	130.2±62.9	438.7±184.3	0.5	24
IL-10	13.7±4.3	28.3±15.3	−0.6	2.5
IL-12p70	16.7±5.2	25.7±11.3	−0.5	2
IL-5	0	34.8±7.5	0	2
IFN-γ	44.9±37.9	16.4±0.6	0.66	2
IL-18	10.3±3.7	1285.2±297.0	0.80	2
GRO/KC	184.3±100.2	522.7±265.1	0.9*	10
TNF-α	0	23.9±7.7	0	10
IL-17	16.6±2.4	17.5±1.5	−0.5	2

Cytokine concentrations in the tissue were normalized to respective bladder weight of each animal. A positive correlation between urine and tissue levels was noticed for GM-CSF, MCP-1, IL-18 and IFN-γ with significant spearman correlation for IL-1β, IL-4 and GRO/KC. The measured Spearman correlation coefficient between levels in tissue and in last voided urine sample for each cytokine was tested for significant difference from 0 by setting a two tailed

^*p≤0.05; n=4–8.