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. 2009 Jan 27;30(2):119–132. doi: 10.1210/er.2008-0025

Figure 3.

Figure 3

Analysis of germ cell DNA content of 14-wk-old male AR+/y (A), S-AR−/y (B), L-AR−/y (C), PM-AR−/y (D), and G-AR−/y (E) mice by using flow cytometry. 1N represents haploid cells, 2N represents diploid cells, and 4N represents tetraploid cells. Compared with AR+/y testis (A), S-AR−/y testis showed 3-fold increase in diploid cells, 2-fold increase in tetraploid cells, and 11-fold reduced haploid cells (B); L-AR−/y testis showed 4-fold increase in tetraploid cells and 2.8-fold reduced haploid cells (C). There were similar distributions of DNA content histogram picks between PM-AR−/y (D), G-AR−/y (E), and AR+/y testis. Histology of testis by hematoxylin and eosin staining in testicular sections from 14-wk-old AR+/y (F), S-AR−/y (G), L-AR−/y (H), PM-AR−/y (I), and G-AR−/y (J) mice. Four to six 14-wk-old mice from individual groups were killed, and testes were excised for histology section. Compared with AR+/y testis (F), S-AR−/y testis showed that decrease of lumen formation in seminiferous tubules as well as germ cell development stopped at diplotene primary spermatocyte (G); L-AR−/y testis showed that decrease of lumen formation in seminiferous tubules as well as germ cell development stopped at round spermatid and no further differentiated elongated spermatid or released spermatozoa can be found (H); PM-AR−/y (I) and G-AR−/y (J) testis showed relatively comparable seminiferous tubule diameters and full range of germ cell development. [Sections of figures were reproduced from Refs. 55 and 56. Copyright 2006, Proceedings of the National Academy of Sciences.] All of these results originated from our previous publications.