Table 1. Primers used for amplification and sequencing of rotavirus genes.

Primer*	Sequence (5′ → 3′)†	Gene‡	Nucleotide position, strand	Amplicon size, bp	Strain	Reference
MDEVP1F	AAT CAC AAT CTG CAG TTC AAA	VP1	68–­89, +	337	Ku	This article
MDEVP1R	AAT GAA TCA GTG TAT TCT TCG	VP1	405–384, –		Ku	This article
MDEVP2F	CTG ACA AAG TGC TAT CAC A	VP2	156–175, +	300	Ku	This article
MDEVP2R	AGG TAA TTG TCT TGG TTC	VP2	456–438, –		Ku	This article
MDEVP3F	TTG CTA GAT TGT CAA ATC GTG	VP3	597–618, +	327	Ku	This article
MDEVP3R	AAT AAG ATG GAG CTG AAC C	VP3	924–905, –		Ku	This article
MDENSP1F	GAG ACC RTC AAC TCC TAC YAA	NSP1	120–141, +	344	Wa	This article
MDENSP1R	ATT GTA AYG TTA TTG GCA T	NSP1	464–445, –		Wa	This article
MDENSP2F	GCT TGC TTT TGT TAT CCT	NSP2	58–76, +	327	Ku	This article
MDENSP2R	ATT TTC CAA ATG TCT AAC AG	NSP2	385–365, –		Ku	This article
MDENSP3F	GCC ACT TCA ACA TTA GAA	NSP3	101–119, +	303	Ku	This article
MDENSP3R	TAC ACT AAA ACA AGC ATT AAG	NSP3	404–383, –		Ku	This article
MDENSP5F	AGC GCT ACA GTG ATG TCT CT	NSP5	10–29, +	337	Ku	This article
MDENSP5R	CCA TTT GAT CGC ACC CA	NSP5	347–330, –		Ku	This article
JRG30	GGC TTT TAA AAG TTC TGT T	NSP4	1–19, +	737	Wa	This article
JRG31	ACC ATT CCT TCC ATT AAC	NSP4	738–721, –		Wa	This article
Con3	TGG CTT CGC TCA TTT ATA GAC A	VP4	11–32, +	876	Ku	(5)
Con2	ATT TCG GAC CAT TTA TAA CC	VP4	887–868, –		Ku	(5)
9con1-L	TA GCT CCT TTT AAT GTA TGG TAT	VP7	37–59, +	896	Wa	Modified from (6)
VP7-Rdeg	GAC GGV GCR ACT ACA TGG T	VP7	933–914, –		Wa	Modified from (7)
VP6-F	GAC GGV GCR ACT ACA TGG T	VP6	747–766, +	379		(8)
VP6-R	GTC CAA TTC ATN CCT GGT G	VP6	1126–1106, –			(8)

*F, forward; R, reverse.
†R, A or G; Y, C or T; V, A, C, or G; N, A, C, G, or T.
‡VP, structural protein; NSP, nonstructural protein.