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. Author manuscript; available in PMC: 2009 Mar 30.
Published in final edited form as: Nat Immunol. 2008 Jul 27;9(9):1055–1064. doi: 10.1038/ni.1641

Fig. 2. PLZF expression is limited to iNKT cells and does not affect the bulk of lymphocyte development.

Fig. 2

(A) Intracellular staining for PLZF expression in B cells and NK cells was done and compared to the same cell types from PLZF deficient mice. Expression of PLZF in iNKT cells is shown as a positive control. (B) Single cell suspensions of thymus and lymph node cells were stained with the indicated monoclonal antibodies. The percentage and phenotype of the major subpopulations of thymocytes and lymphocytes were similar in wild type and PLZF deficient mice, based on staining with CD4, CD8 and TCR. Subtle variations in thymocyte numbers were not reproducible. Only TCR+ lymph node cells are shown. Experiment was repeated approximately 10 times. (C) Frozen thymus sections were stained with Dec205 and with UEA-1 to identify the thymic cortex and medulla. No structural changes to the organ were observed. (D) The frequency of NK cells, DX5+ T cells, and γδ T cells is not affected by the loss of PLZF expression. Data are representative of three independent experiments.