Skip to main content
. Author manuscript; available in PMC: 2009 Mar 30.
Published in final edited form as: Nat Immunol. 2008 Jul 27;9(9):1055–1064. doi: 10.1038/ni.1641

Fig. 6. PLZF deficient iNKT cells do not rapidly produce cytokines.

Fig. 6

(A) Mice (wild type, PLZF deficient, CD1d deficient) were I.P. immunized with 4 gof α-GalCer. Six hours later the spleen was removed, dissociated and stained with the indicated antibodies. Activation of the indicated cell type was measured by upregulation of CD69. (B) Sera were collected from the immunized mice six hours after immunization, diluted 10-fold and ELISAs were done to detect the cytokines IL-4, IFNγ and IL-2. These cytokines were not detected (n.d.) in the PLZF deficient mice or in the CD1d deficient mice. Experiments were done with three animals per group and were repeated twice with nearly identical results. ELISA data represent the means, with standard deviations for the two independent experiments.