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. Author manuscript; available in PMC: 2009 Nov 25.
Published in final edited form as: Mol Cell Endocrinol. 2008 May 21;295(1-2):59–69. doi: 10.1016/j.mce.2008.05.008

Fig. 6. Competitive inhibition of TIF2 and SMRT for altering the properties of GR-regulated gene repression.

Fig. 6

Experiment and work-up are precisely the same as for Fig. 3 except that 20 ng of TIF2 and 20 ng of SMRT plasmids were used with Dex or 1 µM Prog. The average change (± S.E.M.) relative to the control with no added factor for (A) fold repression (Vmax) and (B) percent partial agonist activity of 1 µM of the antiglucocorticoid Prog (left half of figure) and EC50 (right half of figure) from 5–6 independent experiments is plotted. Asterisks on either side of the slash above the data for TIF2 + SMRT refer to the significance compared to GR + TIF2 (left of slash) and to GR + SMRT (right of slash). −P > 0.05, * P < 0.05 (t-test and paired t-test).