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. Author manuscript; available in PMC: 2009 Mar 30.
Published in final edited form as: J Gen Virol. 2008 Jan;89(Pt 1):97–105. doi: 10.1099/vir.0.83226-0

Fig. 6.

Fig. 6

pUS28 requires the G-protein coupling motif, but not the chemokine binding domain for signalling through the PLC-β pathway in HCMV-infected cells. HFFs were infected with increasing m.o.i. (0.03 to 3.0 p.f.u. per cell) using HCMV FLAG–US28/WT or HCMV FLAG–US28/R129A (a), and HCMV FLAG–US28/WT or HCMV FLAG–US28/ΔN (b). Medium containing 1 μCi ml−1 [3H]myoinositol was added at 24 h post-infection and accumulated InsPs were determined at 48 h post-infection using anion-exchange chromatography. The data represent at least four independent experiments performed in duplicate and are presented as a ratio of accumulated inositol phosphates to total 3H incorporation.