Figure 3. Effects of arginase inhibition on DNA synthesis, p21 protein expression, apoptosis and endothelial regrowth after rat carotid artery balloon injury.
A. Representative immunostaining for PCNA (shown in red) in injured carotid arteries 2 days post-injury. B. PCNA labeling index (LI) in the media at 2, 7, and 14 days post-injury. C. PCNA LI in the neointima at 7 and 14 days post-injury. D. Western blot of p21 protein in injured carotid arteries 2 days post-injury. E. TUNEL LI two days post-injury. F. Reendothelialization of carotid arteries 14 days post-injury. Arteries were treated topically with an empty gel, or with gel containing BEC (1mg) or L-OHNA (1mg) immediately after injury. Results are mean ± SEM of 4–6 experiments. *Statistically significant effect of BEC or L-OHNA.