Figure 3.
Typical 13C spectrum of 3.5 mM 1-13C-succinate-d2 hyperpo-larized at pH = 2.9. The chart on the left shows the typical reproducibility achieved; a series of three experiments was conducted within 30 min yielding 17.6 ± 1.4% polarization. Natural abundance ethanol with 207 mM of 13C per carbon site is used as a reference. Spectra are acquired at 4.7 T with a Bruker Avance console and home-built double resonant probe.15