(A) Effect of rif1Δ or rif2Δ mutation on telomere addition at TG162 ends. TG162-HO cells were analyzed by Southern blot as in Fig. 1B.
(B, C) Effect of rif1Δ or rif2Δ mutation on association of Tel1 and Mre11 with TG162 ends. TG162-HO cells expressing Tel1-HA (B) or Mre11-myc (C) were analyzed by ChIP assay as in Fig. 1C.
(D) Effect of tel1Δ mutation on telomere addition at TG162 ends in rif2Δ or rif1Δ rif2Δ mutants. TG162-HO cells were analyzed by Southern blot as in A.
(E) Association of Rif1 with TG81 or TG162 ends. HO, TG81-HO, TG162-HO and TG162-HO rif2Δ cells expressing Rif1-HA were analyzed by ChIP assay as in B.
(F) Association of Rif2 with TG81 or TG162 ends. HO, TG81-HO, TG162-HO and TG162-HO rif1Δ cells expressing Rif2-HA were analyzed by ChIP assay as in B.
(G) Association of Rap1 with TG81 or TG162 ends. HO, TG81-HO, TG162-HO and TG162-HO cells expressing Rap1-HA were analyzed by ChIP assay as in B.