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The binding of synthesized peptides F1, F2, F3 and F4 to glutathione Sepharose 4B beads.
| Peptide | Binding efficiency | |
| Before elution | After elution | |
| F1 | 99.6% | 2.1% |
| F2 | 99.3% | 1.8% |
| F3 | 1.2% | 1.2% |
| F4 | 2.6% | 2.5% |
The binding efficiencies of different peptides were determined according to the ratio of peptide concentrations after the binding and before the binding experiments. Moreover, the peptide binding characteristics were determined again, after the peptides were eluted with the elution buffer.
