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. 2009 Mar 16;10:6. doi: 10.1186/1471-2091-10-6

Table 5.

The binding of F1 and F2 peptides to GST.

Peptide-glutathione Sepharose 4B beads Binding characteristics of GST on peptide-glutathione Sepharose 4B beads
Bmax (site) Kd (pM)
F1 peptide-glutathione Sepharose 4B beads 1.2 156.3
F2 peptide-glutathione Sepharose 4B beads 1.5 114.2

The experiments were performed according to the binding characteristics of GST with F1 peptide-glutathione Sepharose 4B bead complex and F2 peptide-glutathione Sepharose 4B bead complex. The concentrations of bound and free GST were determined after separation of complexes by centrifugation, and the free GST concentration in the supernatant was determined by the Lowry method.