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. 2009 Apr 10;5(4):e1000372. doi: 10.1371/journal.ppat.1000372

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This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

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Kinetics of IgG (upper) and IgM (lower) on the surface of CD4⁺ T cells from ND (left, n = 8) and MD (right, n = 4) macaques are shown graphically. Frozen PBMC samples were stained with antibodies against CD4 (FITC), human IgG, IgM, or IgG₁ isotype control (PE), 7-AAD (PerCP), and CD3 (APC). Percentages of IgG⁺ or IgM⁺ cells in live CD4⁺ T cells (7-AAD⁻CD3⁺CD4⁺) were determined by the Overton cumulative histogram subtraction using mouse IgG₁ isotype control.