TABLE 5.
NONTYPEABLE Haemophilus influenzae ANTIGEN INDUCTION OF HUMAN ALVEOLAR AND BLOOD MACROPHAGE IL-12
| LOS | P6 | P2 | Total OM | |
|---|---|---|---|---|
| Alveolar macrophages | ||||
| Group 1† | 0.0 (0.0)‡ | 0.0 (0.0)‡ | 0.0 (0.0) | 0.0 (0.8)‡ |
| Group 2 | 0.0 (5.6)§ | 0.0 (18.7) | 0.0 (0.0) | 0.0 (4.9)§ |
| Group 3 | 32.9 (226.0) | 63.7 (219.9) | 0.0 (33.9) | 23.3 (145.3) |
| Kruskal Wallis p value | 0.005 | 0.017 | 0.14 | 0.042 |
| Blood macrophages | ||||
| Group 1 | 0.0 (6.4)‡ | 0.0 (22.7)‡ | 0.0 (0.0) | 0.0 (14.8)‡ |
| Group 2 | 0.0 (0.0)§ | 0.0 (18.5)§ | 0.0 (0.0) | 0.0 (7.6)§ |
| Group 3 | 32.5 (94.9) | 78.8 (69.2) | 8.6 (42.8) | 53.6 (116.8) |
| Kruskal Wallis p value | 0.009‖¶ | 0.003¶ | —** | 0.01‖ |
Definition of abbreviations: LOS = lipooligosaccharide; OM = outer membrane.
*Alveolar and blood macrophages of each donor were treated with 0.1 μg/ml of each nontypeable H. influenzae antigen. Values correspond with data in Figure 4. Each value is expressed as median pg/ml (interquartile range).
Group 1, chronic obstructive pulmonary disease; Group 2, exsmokers; Group 3, nonsmokers.
p < 0.05, Group 1 versus Group 3 by Mann Whitney U rank test.
p < 0.05, Group 2 versus Group 3 by Mann Whitney U rank test.
Cumulative pack-years was an independent determinant of total OM induction of IL-12 (p = 0.04) and LOS induction of IL-12 (p = 0.01) for blood macrophages.
Age was an independent determinant of OMP P6 induction of IL-12 (p = 0.04) and of total OM induction of IL-12 (p = 0.03).
p < 0.05, but values were too low for biological significance.