Figure 5.

Immunofluorescence analysis of antibody X156 directed against the titin domain encoded by exon 156. Double-staining with antibodies X156 and T12 revealed greater staining intensity of X156 in diaphragm cryosections from patients with COPD (green, A–B), whereas staining intensity of T12 was similar in those of both patient groups (red, C–D). Bar: 50 μm. Confocal microscopy demonstrated the expected staining pattern of both antibodies: red striations of T12 indicate the location of the sarcomeric Z-lines and green striations of X156 indicate the PEVK regions of the sarcomere (E–F). Bar: 2.5 μm.