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. 2009 Mar 30;106(16):6579–6584. doi: 10.1073/pnas.0813146106

Fig. 4.

Fig. 4.

mRNA and foreign protein accumulation in transplastomic plants. (A) Analysis of gfp, pal, and cpl-1 mRNA accumulation in plants before (selfed) and after (Cre) excision of the transcription block. The ≈1-kb size difference between the major transcripts in the selfed lanes and the corresponding Cre lanes corresponds to the size of the aadA marker and the transcription block. To confirm equal loading, the ethidium bromide-stained agarose gels are also shown. (B) Confirmation of complete removal of the marker gene by Northern blot analysis with an aadA-specific probe. (C) Increase in GFP accumulation by removal of the transcription block. Three micrograms TSP was loaded per lane. For quantitation, 2 samples of purified GFP protein were included. (D) Detection of Pal and Cpl-1 by Coomassie staining of total soluble protein. The large (L) and small (S) subunits of Rubisco and the Pal (P) and Cpl-1 (C) proteins are indicated. Band sizes of the molecular mass marker are given in kDa. (E) Quantitation of Pal and Cpl-1 protein accumulation levels by comparison with a dilution series of purified Rubisco.