Figure 4.

Mitochondrial Oxyblots. Representative Western blot (top) probed with antibody to derived protein carbonyl side groups (i.e., the Oxyblot technique). A second Western blot probed with anti–cytochrome c (bottom blot) served as a loading control. Group mean data for densitometry from this group of blots are presented in the lower graph. LPS administration resulted in a large increase in the protein carbonyl content of a 30-kD protein (shown by arrow, p < 0.001 for group mean data). PEG-SOD administration prevented this increase; p < 0.002 for comparison of LPS to the LPS + PEG-SOD group.