| Fraction | High Mol. Wt. Fraction | Low Mol. Wt. Fraction | Vector only | No ligase added |
| Vector | 1 | 1 | 1 | 1 |
| 5X ligase buffer | 2 | 2 | 2 | 2 |
| T4 ligase (5U/μl) | 1 | 1 | 1 | 0 |
| dH20 | 0 | 0 | 6 | 7 |
| Concatemers | 6 | 6 | 0 | 0 |
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