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. Author manuscript; available in PMC: 2010 Apr 15.
Published in final edited form as: Biosens Bioelectron. 2009 Jan 31;24(8):2604–2610. doi: 10.1016/j.bios.2009.01.029

Figure 1.

Figure 1

Culturing cells with sensing hydrogel micropatterns. (A) HRP-containing PEG hydrogel micropatterns are fabricated on glass substrate. (B) Encapsulation of enzyme molecules does not diminish cell-resistive properties of PEG hydrogel. When incubated with micropatterned substrates macrophages attach next to sensing hydrogel structures. (C) Macrophages release H2O2 upon mitogenic stimulation. Release of this metabolite in the presence of Amplex Red results in an appearance of fluorescence signal in the adjacent PEG hydrogel structures.