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. Author manuscript; available in PMC: 2010 Jan 16.
Published in final edited form as: Biochem Biophys Res Commun. 2008 Dec 4;378(3):662–667. doi: 10.1016/j.bbrc.2008.11.112

Fig. 2.

Fig. 2

Heat-denatured firefly luciferase and CS bound to FG is competent for refolding. (A) Heated mixture of luciferase and FG added into rabbit reticulocyte lysate (RRL) (Δ), heated mixture of luciferase and BSA added into RRL (□), heated luciferase alone added into RRL (○). Error bars represent the standard error from duplicated measurements. (B) Heated mixture of luciferase and FG added into FG solutions, heated mixture of luciferase and FG added into RRL, heated mixture of luciferase and BSA, IgG, transferrin, lysozyme added into RRL. The height of the column represents the highest activity recovered. (C) Reactivation of CS. CS alone (○) or with 0.9 µM FG (●).