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. 2009 Apr 8;4(4):e5108. doi: 10.1371/journal.pone.0005108

Figure 7. Sensitivity to Density & VEGF-Binding Affinity of Interstitial Matrix Sites for VEGF165 & sVEGFR1.

Figure 7

Steady-state sensitivity of VEGF165 (A) and sVEGFR1 (B) distributions in blood (top), normal tissue (middle) and calf tissue (bottom) compartments to the VEGF165-binding affinity (Kd(M,V)) and densities ([ECM]; [BM]) of interstitial matrix sites. At control: Kd(M,V) = 23.8 nM; [ECM] = 20 µM; [BM] = 0.75 µM. Concentrations of soluble species (e.g., free VEGF165, free sVEGFR1, sVEGFR1-VEGF165) and surface VEGFR occupancies (e.g., VEGF165-VEGFR1, VEGF165-VEGFR2, VEGFR2-VEGF165-NRP1) were completely insensitive; whereas the sizes of matrix-bound reservoirs of VEGF165 and sVEGFR1 (e.g., VEGF165-ECM, sVEGFR1-PBM) were greatly affected. ‘Kd(M,V)’ = dissociation constant between interstitial matrix sites and VEGF165; ‘[ECM]’ and ‘[BM]’ = densities of binding sites for VEGF or sVEGFR1 in extracellular matrix and basement membranes respectively’; ‘Ctrl’ = control; ‘PBM’ = parenchymal BM; ‘EBM’ = endothelial BM; V165 = VEGF165; ‘R1’ = VEGFR1; ‘sR1’ = sVEGFR1; ‘R2’ = VEGFR2.