Figure 3. Assesment of CRAd lytic activity in vitro on non-cancerous human cells.

(A) Lysis of normal melanocytes by Ad5-wt but not by the CRAd. (B) HaCaT adult kerantinocytes and normal adult fibroblasts sensitivity to Ad-wt but not to the CRAds. (C) Lytic activity of the Adwt and the CRAd on WI-38 fibroblasts and HMEC-1 cells (Of note is that HMEC-1 cells detached from the well in the absence of virus). (D) Reduced HMEC-1 viability after infection with Ad-(I)F512-TK (5×10⁶ vp/ml) followed by GCV. Remaining viable cells were stained as described. ***P<0.001 (one-way ANOVA follow by a Tukey multiple comparison test).