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. Author manuscript; available in PMC: 2010 Mar 6.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Jan 24;380(2):349–354. doi: 10.1016/j.bbrc.2009.01.085

Fig. 4.

Fig. 4

Effects of Ikkβ deletion on growth-signaling systems in cultured hepatocytes. (A) Heightened IkkβΔhep sensitivity to mitogens. Duplicate wells of cells plated (N0 = 1.5 × 105) into 24-multiwell plates received serum-free plating medium (0-dose), TGFα (top panel) or TNFα (bottom panel). Curves are differences between the average AUC ± S.E[each treatment] minus the average AUC ± S.E[0-dose] integrated over d1–d9 cell counts. Average AUC values[0-dose] subtracted from each AUC[ligand dose] were 42026 (IkkβF/F) and 61023 (IkkβΔhep; see Suppl. Table 3). Intergenotypic P values between curves: TGFα, P < 0.0005; TNFα, P < 0.03. (B) Augmented IkkβΔhep cytoskeletal, ECM, basement membrane and integrin mRNA expression. Curves are averages, 3 platings (N0 = 3 × 105); N = 6 dishes/primer pair/point (sister culture growth was similar to Fig. 2A). Primer pair results were combined (Suppl. Table 1), except for nidogen (N = 6 dishes/primer pair 1/point; N = 4 dishes, primer pair 2/point). Intergenotypic P values d0–d6: fibronectin (P < 0.07); collagen I (P < 0.003), collagen III (P < 0.0003); nidogen (P < 0.02); β-actin (P < 0.03); integrin B1 (P < 0.0001).