Table 1. Summary of PRV neuroinvasion by examination of trans-synaptically labeled neurons.
| WT | C26A | Rev (C26) | 1∶1d | PRV-152 | |
| Anterograde (SC)a | |||||
| Intravitrealb | 1/5 (20%) | 4/15 (27%) | n/d | n/d | n/d |
| Retrograde (EW)a | |||||
| Intravitrealb | 5/5 (100%) | 0/15 (0%)c | n/d | n/d | n/d |
| Anterior Chamberb | 3/3 (100%) | 0/4 (0%) | 3/3 (100%) | 3/3 (100%)e | 4/4 (100%) |
| Retrograde (RGC)a | |||||
| SCNb | n/d | 2/2 (100%) | n/d | n/d | n/d |
| Retrograde (CNS)a | |||||
| Eyelidb | n/d | 1/11 (9%) | n/d | n/d | 10/11 (91%) |
a
site imaged.
b
site injected.
c
one animal showed fluorescence signal in the oculomotor nucleus.
d
1∶1 mixture of PRV-152 (Bartha; green fluorescence) and PRV-GS1652 (C26A; red fluorescence).
e
all 3 animals emitted red and green fluorescence in the EW indicative of PRV-152 and PRV-GS1652 co-infection.
SC superior colliculus.
EW Edinger-Westphal nucleus.
RGC retinal ganglion cells.
CNS central nervous system.
WT virus encoding wild-type pUL36.
C26A virus encoding C26A mutant isoform of pUL36.
Rev revertant of C26A virus (encodes wild-type pUL36).
n/d no data.