Figure 2. DNA methylation and histone acetylation do not alter p110δ expression in mouse L929 fibroblasts.

(Top panel) L929 cells were treated with 5′-azacytidine (5AC,;5 µM) for 72 h and/or trichostatin A (TSA; 100 nM) for 6 h, with or without 6 h co-treatment with TNF (100 IU/ml)). mRNA levels of p110δ, IL-6 and IFN-β were quantified by real time RT-PCR. Samples were normalised for GAPDH and are relative to p110δ mRNA amounts in the unstimulated samples (set as 1). Shown is the average of three independent experiments). (Lower panel) Representative immunoblot (of three) of p110δ protein.