FIG. 5.
Comparative intracellular and extracellular activities of different antibiotics against SCVs exposed for the indicated times at fixed extracellular concentrations (total drug, corresponding to the human Cmax [Table 1]). Antibiotics are ordered from classical first-line drugs to more recent alternatives and drugs under development. No data were obtained for control cells at 72 h (massive infection and cell death). The graphs show the change in the number of CFU over time (Δlog CFU from the initial inoculum) per mg of cell protein (intracellular) or per ml of culture medium (extracellular). Data are means ± standard deviations (n = 3); most error bars are smaller than the symbols. The dotted lines highlight changes of −1, −2, and −3 logs to facilitate comparisons between drugs. The limit of detection is set at −4.5 logs. Drug abbreviations are given in footnote a to Table 1. Contr, control.