FIG. 4.

Nine-day incubation of genotype 1b replicon cells with TMC435350 alone, IFN-α alone, and TMC435350 in combination with IFN-α. Huh7-Luc replicon cells were harvested after 3, 6, or 9 days of treatment, and RNA levels were assessed by qRT-PCR. The normalized (host RPL13A) HCV RNA levels of treated replicon cells relative to untreated cells are plotted on a log₁₀ scale. □, 0.5 U/ml IFN-α; ▵, 5 nM TMC435350; ▴, 5 nM TMC435350 plus 0.5 U/ml IFN-α; ○, 50 nM TMC435350; •, 50 nM TMC435350 plus 0.5 U/ml IFN-α. Each point represents the mean value of three independent experiments.