FIG. 4.

LbMT-GFP partially rescues MLF uptake in LdMT^−/− parasites. L. donovani LdMT^−/− promastigotes were transfected with LdMT-GFP or LbMT-GFP. (A) [¹⁴C]MLF internalization was measured after 60 min of incubation at 28°C and is expressed as a percentage of the internalization by wild-type L. donovani. Results are shown as the mean ± standard deviation of three independent experiments. (B) Affinity-purified biotinylated proteins from the surface of live parasites were analyzed by immunoblotting with anti-GFP and anti-LdRos3 antibodies. Western blot assays representative of at least three independent experiments are shown. The positions of molecular mass markers (kilodaltons) are indicated on the left.