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Iex dependence of the flicker rate (■, □) and detected fluorescence emission rates (●,○) (photons/second/molecule) in T203Y at 514-nm (filled) in comparison with 488-nm (open) excitation. The 488-nm intensity was scaled by a factor of 1/2 to account for the difference in molecular extinction coefficient at 514 nm. In contrast to the fluorescence emission rates, saturation is not observed for the blinking rates, although the slope decreases slightly at very high Iex.
