TABLE 1.
Effects of rRNA mutations on the susceptibility of E. coli to macrolide antibiotics
| Mutationa | MIC (μg/ml)
|
||
|---|---|---|---|
| Erythromycin | Telithromycin | K-1325 | |
| Wild type | 128 | 16 | 8 |
| A2058G | >500 | >150 | >250 |
| U2609C | 128 | 64 | 8 |
a
Wild-type or mutant RNAs were expressed in the TA531 strain of E. coli (39) from plasmid-borne genes that were the single source of rRNA in the cell. Wild-type cells contained plasmid pKK3535 (6), the A2058G mutant contained plasmid pSTL102 (35), and the U2609C mutant contained plasmid pKK3535(2609C) (11).