TABLE 1.
Strains used in this study
| Strains | Genotype/purpose description | Reference, source, and/or constructiona |
|---|---|---|
| W168 | trpC2 | BGSC no. 1A1 |
| CU1065 | W168 trpC2 attSPß | 59; Lab stock |
| HB5121 | W168 liaIHGFSR::spc | LFH-PCR → W168 |
| HB0934 | CU1065 liaGFSR::kan | 43 |
| HB0920 | CU1065 liaH::kan | 43 |
| HB0935 | CU1065 liaIH::tet | 43 |
| HB0933 | CU1065 liaR::kan | 43 |
| HB5337 | CU1065 mprF::kan | 52 |
| HB5123 | CU1065 liaIH::tet mprF::kan | HB5337 chr DNA → HB0935 |
| HB0919 | CU1065 pspA::cat | 4 |
| HB5124 | CU1065 liaH::kan pspA::cat | HB0919 chr DNA → HB0920 |
| HB5125 | CU1065 liaIH::tet pspA::cat | HB0919 chr DNA → HB0935 |
| HB5126 | CU1065 liaIH::tet mprF::kan pspA::cat | HB0919 chr DNA → HB5123 |
| HB0938 | CU1065 yhcYZ::cat | T. Mascher, unpublished |
| HB5127 | CU1065 liaH::kan yhcYZ::cat | HB0938 chr DNA → HB0920 |
| HB0031 | CU1065 sigM::kan | 12 |
| HB0020 | CU1065 sigW::mls | 7 |
| HB7007 | CU1065 sigX::spc | 27 |
| HB0097 | CU1065 sigX::spc sigM::kan | 9 |
| HB0096 | CU1065 sigW::mls sigM::kan | 11 |
| HB0030 | CU1065 sigX::spc sigW::mls | M. Cao, unpublished |
| HB0982 | CU1065 sigM::kan sigW::mls sigX::spc | 42 |
| HB5128 | CU1065 liaH::kan sigW::mls | HB0020 chr DNA → HB0920 |
| HB5129 | CU1065 liaH::kan pspA::cat sigW::mls | HB0020 chr DNA → HB5124 |
| HB5130 | CU1065 sigX::spc sigM::kan liaIH::tet | HB0935 chr DNA → HB0097 |
| HB5131 | CU1065 sigM::kan sigW::mls sigX::spc liaIH::tet | HB0935 chr DNA → HB0982 |
| HB5132 | CU1065 sigW::mls pspA::cat | HB0919 chr DNA → HB0020 |
| HB5133 | CU1076 sigM::kan sigW::mls sigX::spc pspA::cat | HB0919 chr DNA → HB0982 |
| HB5134 | W168 des::spc | LFH-PCR → W168 |
| HB5135 | W168 des::spc pspA::cat | HB0919 chr DNA → HB5134 |
| HB5346 | CU1065 ugtP::mls | 52 |
| HB5361 | CU1065 pssA::spc | 52 |
| HB5347 | CU1065 ywnE::tet | 52 |
| HB5343 | CU1065 psd::mls | 52 |
| HB5350 | CU1065 mprF::kan ugtP::mls | 52 |
| HB5388 | CU1065 mprF::kan pssA::spc | 52 |
| HB5344 | CU1065 psd::mls mprF::kan | 52 |
| HB5136 | CU1065 mprF::kan pspA::cat | HB0919 chr DNA → HB5337 |
| HB5363 | CU1065 PxylA-mprF at the thrC locus, Spcr | L. I. Salzberg, unpublished |
| BFA2809 | W168 trpC2 pgsA::pMutin4 | 2 |
| HB0950 | CU1065 attSPß2Δ2::PliaI-cat-lacZ | 44 |
| HB0070 | CU1065 sigM::kan SPß (PM-cat-lacZ) | 9 |
| HB7022 | CU1065 SPß7019 (PX-cat-lacZ) | 27 |
| HB0050 | CU1065 SPß::PsigW-cat-lacZ | 28 |
| HB0021 | CU1065 sigW::mls SPß::PsigW-cat-lacZ | 12 |
a
Long-flanking homology PCR (LFH-PCR) was applied as described previously to construct some of the deletions, using the primers listed in Table 2.