TABLE 2.
Oligonucleotides used in this study
| No. | Name | Sequencea |
|---|---|---|
| 2443 | Lia-Do-Fwd(spec) | 5′-CGTTACGTTATTAGCGAGCCAGTCGCGGCTGCTATTTGTTTGCGCC-3′ |
| 2444 | Lia-Do-Rev | 5′-GGAGGGCTCTTCATCTGATCCG-3′ |
| 2445 | Lia-Up-Rev(spec) | 5′-CAATAAACCCTTGCCCTCGCTACGAAAAACGCCATGCACGAGGC-3′ |
| 2446 | Lia-Up-Fwd | 5′-GCTGTCACATTATGCGGCGC-3′ |
| 3197 | Des-Up-Fwd | 5′-CCCACTTCTCAACATTTGCAA-3′ |
| 3198 | Des-Up-Rev(spec) | 5′-CGTTACGTTATTAGCGAGCCAGTCCGACTTGCTTTGTCAGCTGT-3′ |
| 3199 | Des-Do-Fwd(spec) | 5′-CAATAAACCCTTGCCCTCGCTACGGTTTGTGTCATT TGGGCTAT-3′ |
| 3200 | Des-Do-Rev | 5′-CCCAAGCGTATCATGGAGAT-3′ |
a
Sequences complementary to antibiotic resistance cassettes for LFH-PCR are underlined.