TABLE 4.
MIC of B. subtilis mutants and strains with altered membrane composition, or deletion of transcriptional regulators
| Strain/deletion mutanta | DAP MIC (μg/ml)b |
|---|---|
| W168 | 1.0 |
| CU1065 | 1.0 |
| LiaR-regulated | |
| liaIHGFSR | 0.3 |
| liaH | 0.4 |
| liaIH | 0.4 |
| liar | 0.5 |
| liaF | 1.0 |
| liaGFSR | 0.5 |
| liaIH mprF | 0.2 |
| liaH yhcYZ | 0.2 |
| yhcYZ | 0.8 |
| ECF σ-regulated | |
| sigM | 0.8 |
| sigW | 0.8 |
| sigX | 1.0 |
| sigXM | 0.6 |
| sigWM | 0.7 |
| sigXW | 0.9 |
| sigMWX | 0.6 |
| pspA | 1.0 |
| LiaR/ECF σ-regulated | |
| liaH sigW | 0.3 |
| liaH pspA sigW | 0.2 |
| liaH pspA | 0.2 |
| liaIH pspA | 0.2 |
| liaIH mprF pspA | 0.2 |
| sigXM liaIH | 0.2 |
| sigMWX liaIH | 0.2 |
| sigW pspA | 0.8 |
| sigMWX pspA | 0.5 |
| Membrane alterations | |
| W168 at 25°C | 0.7 |
| des at 25°C | 0.4 |
| des | 0.9 |
| des pspA | 0.9 |
| ugtP | 0.9 |
| pssA | 1.0 |
| ywnE | 1.0 |
| psd | 1.0 |
| mprF | 0.5 |
| mprF ugtP | 0.6 |
| mprF pssA | 0.9 |
| psd mprF | 0.9 |
| mprF pspA | 0.5 |
| mprF overexpression | 1.3 |
| pgsA depletion | 8.0 |
| IPTG-induced pgsA | 1.0 |
a
liaIHGFSR and des mutants are derived from strain W168. Other mutants are derived from strain CU1065.
b
MIC was determined by liquid growth inhibition experiments. Data represent the mode of the lowest daptomycin (DAP) concentration that led to complete growth inhibition (minimum of triplicates).