FIG. 4.

Effect of rifampin (Rif.) on the activation of MAPK and STAT-1. Cells were incubated with IL-1ß, TNF-α, and IFN-γ (Mix) in the absence or the presence of the indicated concentration of rifampin (Rif.). Cytoplasmic extracts were prepared at the indicated times and were subjected to Western immunoblotting with antibodies specific for the phosphorylated forms of ERK1/2, JNK, p-38, and STAT-1 (P-Erk, P-JNK, P-p38, and P-STAT-1, respectively). The results were compared to those obtained with ERK1/2, JNK, and p38. Data from one of three experiments with similar results are presented.