FIG. 5.

Downregulation of PPARγ expression by rifampin (Rif.). A Western blot analysis of the level of PPARγ expression in A549 cells was performed 24 h after treatment with medium alone; IL-1ß, TNF-α, and IFN-γ (Mix; 100 ng/ml each); and the mix with different doses of rifampin (Rif). The level of PPARγ expression was then normalized to the level of β-actin expression. (a) A representative blot from one of five experiments with similar results is shown. (b) Results of densitometric analysis in five experiments. *, P < 0.001 for the mix versus the mix plus rifampin.