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. 2009 Jan 21;53(4):1299–1304. doi: 10.1128/AAC.01358-08

FIG. 2.

FIG. 2.

Primer design for amplification of the AHBA synthase gene from S. platensis MA7327 genomic DNA. (a) Primary sequence alignment of GriH, the AHBA synthase from Streptomyces griseus (17), and two highly similar open reading frames from the genome sequence of Frankia sp. strain CCI3 (strain Francci3_2069, GenBank accession no. YP_48177; strain Francci3_4026, GenBank accession no. YP_483283). (b) Primers ptmForward and ptmReverse were designed to amplify an ∼730-bp internal fragment. (c) PCR amplification of an internal fragment of the AHBA synthase gene by use of the annealing temperature to control the specificities of the primers resulted in a single product with the predicted size of 730 bp (lane 2) as determined by comparison to 1 kb Plus DNA Ladder (lane 1) (Invitrogen, Carlsbad, CA).