TABLE 1.
Strains of serovar Typhimurium and plasmids used in this study
| Strain or plasmid | Genotypea or relevant characteristics | Defect(s) | Source or reference |
|---|---|---|---|
| SR-11 wild type | gyr-1816 | None | 11 |
| SR-11 ΔaceA | gyr-1816 ΔaceA::cat | Isocitrate lyase | 47 |
| SR-11 ΔsucCD | gyr-1816 ΔsucCD::cat | Succinyl-CoA synthetase | 47 |
| SR-11 ΔsucCD ΔaceA | gyr-1816 ΔsucCD ΔaceA::cat | Succinyl-CoA synthetase, isocitrate lyase | This study |
| SR-11 ΔfumAC | gyr-1816 ΔfumAC::cat | Fumarase A, fumarase C | This study |
| SR-11 ΔdcuB ΔfumB | gyr-1816 ΔdcuB ΔfumB::cat | DcuB dicarboxylate antiporter, fumarase B | This study |
| SR-11 ΔfumAC ΔdcuB ΔfumB | gyr-1816 ΔfumAC::cat ΔdcuB ΔfumB | DcuB dicarboxylate antiporter, fumarase A, fumarase C, fumaraseB | This study |
| “Restored” SR-11 ΔdcuB ΔfumB | gyr-1816 ΔdcuB ΔfumB | DcuB dicarboxylate antiporter, fumarase B | This study |
| SR-11 ΔsfcA ΔmaeB | gyr-1816 ΔmaeB ΔsfcA::kan | Both malate oxidoreductases | 47 |
| SR-11 Δmdh | gyr-1816 Δmdh::cat | Malate dehydrogenase | 47 |
| SR-11 ΔsfcA ΔmaeB Δmdh | gyr-1816 ΔsfcA ΔmaeB Δmdh::cat | Both malate oxidoreductases, malate dehydrogenase | This study |
| “Restored” SR-11 ΔsfcA ΔmaeB | gyr-1816 ΔsfcA ΔmaeB | Both malate oxidoreductases | This study |
| Plasmids | |||
| pKD3 | Template plasmid; contains chloramphenicol resistance cassette flanked by FLP recombinase target sites; bla cat | 12 | |
| pKD4 | Template plasmid; contains kanamycin resistance cassette flanked by FLP recombinase target sites; bla kan | 12 | |
| pKD46 | Temperature-sensitive plasmid; contains arabinose-inducible phage λ red recombinase gene for linear DNA exchange; bla | 12 | |
| pCP20 | Temperature-sensitive plasmid; contains FLP recombinase gene for removal of antibiotic resistance cassetes; bla cat | 12 |