TABLE 2.
Primers used in this study
| Use and name | Sequence (5′ → 3′)a | Restriction site |
|---|---|---|
| Cotranscription assay | ||
| #44 | GCCATGTTAACTTAATTCCTGTCAACC | |
| #107 | CGCAACCAATTTTCAGCTTGATG | |
| #108 | ACAGATAAACGTGTGAGTCCAGA | |
| #109 | CTTCTTCATCAACATCGATCATACTTAC | |
| #110 | GAATCGGTAGATGTACCATACACTG | |
| #46 | TCGACCTGTCTTCAAAATGGCA | |
| Recombinant proteins | ||
| STP-F | CTACGGCCATATGCTAGAGGCACAATTTTTTACTGATACTGGAC | NdeI |
| STP-R | CCGCGGATCCTCATACTTTATCACCTTCAATAGCCG | BamHI |
| STK-F | CTACGGCCATATGATAGGTAAAATAATAAATGAACG | NdeI |
| STK-R | CCGCGGATCCTTAAACATCATCATAGCTGACTTCTTTTTC | BamHI |
| Deletion strains | ||
| AF | GCGGATCCCATAAAGCAGGAGAAGTTGCAAG | BamHI |
| AR | CTAGCTAGCTCATACTTTATCACCTTCAATAGC | NheI |
| BF | CTAGCTAGCAATTGAAGTAAATGTACCGAGG | NheI |
| BR | TGCCATGGCACTTACCGACACTGATTGACCAC | NcoI |
| catF | CGGCTAGCCGTTGACTTTTAAAAAAGGATTGATTC | NheI |
| catR | CGGCTAGCCTTATAAAAGCCAGTCATTAGGC | NheI |
| #33 | GGGGACCACTTTGTACAAGAAAGCTGGGTGAAACAGATTTTTGAATGGTTATATCAA | |
| #34 | GTTTCACCGCGGGTTTCTACTTGTCGTTCCTTTGC | SacII |
| #58 | GTTTCACCGCGGAGTATGATAGGTAAAATAATAAATG | SacII |
| #59 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCTGGCTTTTGGAATTGCTGATGATGAG | |
| #35 | GTTTCACCGCGGATAATTGAAGTAAATGTACCGAG | SacII |
| #36 | GGGGACAAGTTTGTACAAAAAAGCAGGCTCCATATCGATTTAATTCAAGAA | |
| Complementation | ||
| #73 | CGCGGATCCCTTGTGGTCAATTAAGAGCA | BamHI |
| #65 | CCGGAATTCTCATACTTTATCACCTTCAATAG | EcoRI |
| #66 | CCGGAATTCTTAAATATCATCATAGCTGACTTC | EcoRI |
| #87 | CTACGGCCATATGTTGTCTTTACCTCGTTTCTACTTGTCG | NdeI |
| #88 | CTACGGCCATATGATGATAGGTAAAATAATAAATGAACG | NdeI |
a
Restriction sites are in bold, and att recombination sites are in italics.