FIG. 2.

Effect of IFN-γ on TLR2 expression on macrophages. Peritoneal macrophages were incubated in triplicate with IFN-γ, and the TLR2 surface expression was determined by flow cytometry. (A) The TLR2 expression levels on macrophages incubated in medium alone (gray peak) or in the presence of IFN-γ (black peak) were determined. Unstained cells were also used as a control (unshaded peak). (B) Mean plus highest and lowest values for normalized mean fluorescence intensity (MFI) showing elevated TLR2 expression after incubation with IFN-γ. (C) Western blot showing elevated TLR2 expression in macrophages after incubation with OvAg or Pam₃Cys in the presence or absence of IFN-γ.