FIG. 4.

Effect of IFN-γ on CXC chemokine production by macrophages and corneal fibroblasts. (Upper panels) Macrophages were isolated from the peritoneal cavity of C57BL/6 mice and, after an overnight resting phase, stimulated for 18 h with either IFN-γ, 2 μg of OvAg/μl, or both IFN-γ and 2 μg of OvAg/μl. The production of CXCL1/KC and CXCL2/MIP-2 was determined by ELISA. (Lower panels) MK-T1 corneal fibroblasts were stimulated in a similar manner, and CXCL1/KC and CXCL5/LIX were quantified by ELISA. The data are means ± the SEM and are representative of two repeat experiments for each cell type. ns, no significant difference.